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Published on: October 10, 2011
In vitro transcription of a whole gene on a surface-coupled template
Jenny Steffen1, Markus von Nickisch-Rosenegk, Frank F Bier
1Fraunhofer Institute for Biomedical Engineering, Dept. Molecular Bioanalytics and Bioelectronics, Arthur-Scheunert-Allee 114-116, 14558, Nuthetal, Germany. jenny.steffen@ibmt.fhg.de
Abstract:
An artificial gene was constructed combining the T7 promoter and terminator with the EGFP-gene from the plasmid pEGFP. The functionality of the construct was shown by in vitro translation. The gene-construct was immobilised on a planar glass surface. The transcription was performed on the immobilised gene and mRNA was determined by RT-PCR. Multiple use of the immobilised gene was demonstrated.
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