Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

New method to detect histone acetylation levels by flow cytometry.

Simona Ronzoni1, Mario Faretta, Marco Ballarini

  • 1European Institute of Oncology, Milan, Italy.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|May 26, 2005
PubMed
Summary

A new flow cytometry method detects histone acetylation changes, crucial for understanding gene regulation and cancer. This technique validates histone deacetylase (HDAC) inhibitor treatments in patients.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantitative single-cell analysis of PML-RARα oncogene-induced DNA damage along cell cycle progression.

Methods (San Diego, Calif.)·2026
Same author

Spatiotemporal atlas of pro-inflammatory NF-κB and anti-inflammatory STAT6 signaling using reporter mice during mRNA vaccination.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same author

First-in-Class Covalent Inhibitors of PFKFB3: Discovery and Characterization in PDAC Models.

Journal of medicinal chemistry·2026
Same author

The normal human lymph node cell classification and landscape defined by high-dimensional spatial proteomics.

PloS one·2026
Same author

Mapping Genomic Heterogeneity in Pediatric and Adolescent-Young Adult Sarcomas: Insights from the Italian SAR-GEN2016 and SAR-GEN_ITA Prospective Multicenter Trials.

Cancer research communications·2026
Same author

Neonatal genetic screening of Glucose-6-phosphate dehydrogenase deficiency through next-generation sequencing.

British journal of haematology·2026

Area of Science:

  • Epigenetics
  • Molecular Biology
  • Cell Biology

Background:

  • Histone acetylation is a key epigenetic modification regulating gene expression and chromatin structure.
  • Dysregulation of histone acetylation mechanisms is implicated in cancer development.
  • Maintaining proper levels of histone acetylation is vital for normal cellular function.

Purpose of the Study:

  • To develop and validate a novel flow cytometry technique for detecting histone acetylation.
  • To investigate the dynamic changes in histone acetylation during the cell cycle.
  • To assess the efficacy of histone deacetylase (HDAC) inhibitors in vivo and in clinical settings.

Main Methods:

  • Development of a flow cytometry assay using a monoclonal antibody specific for acetylated histone tails.

Related Experiment Videos

  • Bivariate analysis of histone acetylation levels and DNA content to study cell cycle-dependent chromatin organization.
  • Monitoring histone acetylation and transcription during differentiation induced by retinoic acid and HDAC inhibitors.
  • Analysis of patient blood samples to evaluate HDAC inhibitor effects in vivo.

    • Main Results:

    • The flow cytometry method successfully detected histone acetylation modifications induced by HDAC inhibitors in patient blood samples.
    • Histone acetylation levels exhibited a reproducible increase during the DNA replication phase and decreased at G2M, correlating with transcriptional activity.
    • The technique demonstrated its clinical utility in monitoring HDAC inhibitor treatment efficacy.

    Conclusions:

    • Multiparameter flow cytometry analysis of histone acetylation offers a rapid and reliable tool for diagnosing and evaluating treatment response in clinical trials involving HDAC inhibitors.
    • This method facilitates the assessment of molecular markers, DNA ploidy, and cell cycle kinetics.
    • The developed protocol is validated for clinical use in monitoring epigenetic modifications and treatment efficacy.