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Updated: Aug 17, 2026

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes
Published on: February 14, 2020
Genetic and molecular analysis of the Enhancer (En) transposable element system of Zea mays
A Pereira1, Z Schwarz-Sommer, A Gierl
1Max-Planck-Institut fur Züchtungsforschung, 5000 Köln 30, FRG.
Abstract:
A newly isolated, unstable mutation wx-844::En-1 of Zea mays was proven to be caused by the insertion of the autonomous transposable element En into the Waxy (Wx) gene. Molecular analysis revealed that En-1 is 8.4 kb long, has a 13-bp long perfect inverted repeat at its termini and generates a 3-bp target site duplication. En-1 is integrated into an intron located approximately in the middle of the transcribed region of the Wx gene. Structural evidence is presented indicating that a receptor component (Inhibitor) can arise by internal deletion of an autonomous En element.
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