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Updated: Aug 17, 2026

Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies
Published on: January 10, 2025
[Changes in functional state of the low-molecular G-proteins of platelets in type 1 diabetes mellitus]
Abstract:
Platelets from healthy donors and insuline dependent patients with type 1 diabetes mellitus were examined for proteins specifically interacting in vitro with GST-fused constitutively active (Val12) forms of small GTPases of Rac, Rho and Cdc42. Differential changes in pattern of proteins which bind to these G-proteins in diabetic platelets have been revealed. Obtained results suggest that signalling pathways mediated by Rho GTPases are altered under type I diabetes mellitus. Such changes of actin cytoskeleton regulation may contribute to the higher level of platelet aggregation, which prove to be the etiological background of the late diabetes complications.
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