A rapid and simplified ELISA using whole blood samples of Schistosoma japonicum-infected rabbits

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Isolation and Characterization of Extracellular Vesicles from Adult Schistosoma japonicum
07:37

Isolation and Characterization of Extracellular Vesicles from Adult Schistosoma japonicum

Published on: May 22, 2018

Yoshinori Hirose1, Masashi Kirinoki, Yuichi Chigusa

  • 1Department of Tropical Medicine and Parasitology, Dokkyo University School of Medicine, Mibu, Shimotsuga, Tochigi, Japan.

The Southeast Asian Journal of Tropical Medicine and Public Health
|May 27, 2005
PubMed

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.