Related Experiment Video

Updated: Aug 17, 2026

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Differential regulation of survivin by p53 contributes to cell cycle dependent apoptosis

Yan Jin1, Yong Wei, Lei Xiong

  • 1Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.

Cell Research
|May 27, 2005
PubMed

Insights

Tripchlorolide (TC) up-regulates p53 in HeLa cells, affecting apoptosis differently based on cell cycle stage. p53 differentially regulates survivin, influencing cell death susceptibility during early versus middle S-phase.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Cancer Research

Background:

  • Cell-cycle checkpoints and apoptosis are crucial in regulating cell fate.
  • The p53 tumor suppressor protein plays a significant role in both processes.
  • Human papillomavirus (HPV) oncoproteins E6/E7 can disrupt normal cell cycle control and apoptosis.

Purpose of the Study:

  • To investigate the effect of the anti-tumor reagent tripchlorolide (TC) on HPV-infected HeLa cells.
  • To elucidate the role of p53 and its regulation of downstream genes, such as survivin, in response to TC treatment at different cell cycle phases.

Main Methods:

  • Treatment of HeLa cells with tripchlorolide (TC).
  • Analysis of p53 expression and stabilization.
  • Assessment of apoptosis susceptibility at different cell cycle stages (early vs. middle S-phase).
  • Investigation of survivin gene expression regulated by p53.

Main Results:

  • TC inhibited HPV E6/E7 oncogene expression and up-regulated p53 in HeLa cells.
  • Cells in early S-phase were more susceptible to apoptosis than middle S-phase cells upon TC treatment, despite similar p53 stabilization.
  • p53 up-regulated survivin in middle S-phase cells but down-regulated it in early S-phase cells.

Conclusions:

  • The differential regulation of survivin by p53 at distinct cell cycle phases leads to varied cellular responses to apoptosis inducers.
  • Cell cycle stage-specific modulation of gene expression by p53 is a critical determinant of apoptosis outcome.

More Related Videos

Knockdown of FAM83A to Verify Its Role in Cervical Cancer Cell Growth and Cisplatin Sensitivity04:20

Knockdown of FAM83A to Verify Its Role in Cervical Cancer Cell Growth and Cisplatin Sensitivity

Published on: February 9, 2024

Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells10:33

Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells

Published on: June 16, 2019

Related Concept Videos

Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
Molecular Factors Affecting Cell Division01:27

Molecular Factors Affecting Cell Division

Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...