Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Assays for proteasome assembly and maturation.

R Jürgen Dohmen1, Markus K London, Christoph Glanemann

  • 1Institute for Genetics, University of Cologne, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|May 27, 2005
PubMed
Summary

This study presents novel methods to track proteasome assembly and maturation in bacteria and eukaryotes. These techniques analyze assembly intermediates and mature proteasomes, aiding research into protein degradation pathways.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structural transitions in the stepwise assembly of proteasome core particles.

Nature communications·2026
Same author

Ubiquitin precursor with C-terminal extension promotes proteostasis and longevity.

Molecular cell·2025
Same author

The α5-α6-α7-Pba3-Pba4 Complex: A Starting Unit in Proteasome Core Particle Assembly.

Biomolecules·2025
Same author

Structural roles of Ump1 and β-subunit propeptides in proteasome biogenesis.

Life science alliance·2024
Same author

Hsp70 and Hsp110 Chaperones Promote Early Steps of Proteasome Assembly.

Biomolecules·2023
Same author

Hypusinated eIF5A Promotes Ribosomal Frameshifting during Decoding of ODC Antizyme mRNA in <i>Saccharomyces cerevisiae</i>.

International journal of molecular sciences·2022

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • The 20S proteasome is a crucial protease found across all life forms.
  • Eukaryotic 26S proteasomes, comprising 20S proteasomes and 19S activator complexes, degrade ubiquitylated proteins.
  • Proteasome biogenesis involves gene expression, assembly, and maturation, including autocatalytic processing of beta subunits.

Purpose of the Study:

  • To describe novel methods for tracking proteasome assembly and maturation.
  • To analyze proteasome intermediates and mature forms in both bacterial and eukaryotic cells.
  • To investigate the kinetics of proteasome assembly and maturation processes.

Main Methods:

  • Gel filtration and immunoblotting techniques were employed.
  • Analysis was performed using sodium dodecyl sulfate (SDS)- and native polyacrylamide gel electrophoresis (PAGE).

Related Experiment Videos

  • Pulse-chase experiments tracked beta subunit maturation and Ump1 degradation to monitor assembly kinetics.

    • Main Results:

    • The study successfully established methods to analyze proteasome assembly intermediates and mature forms.
    • Kinetics of proteasome assembly were monitored through the maturation of beta subunits and degradation of Ump1.
    • These methods are applicable to both bacterial and eukaryotic cellular systems.

    Conclusions:

    • The developed methods provide a robust approach to study proteasome biogenesis.
    • Understanding proteasome assembly and maturation is key to comprehending protein homeostasis.
    • These techniques facilitate further research into the regulation of proteasome function.