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Related Experiment Videos

c-abl has a sequence-specific enhancer binding activity.

R Dikstein1, D Heffetz, Y Ben-Neriah

  • 1Department of Molecular Genetics and Virology, Weizmann Institute of Science, Rehovot, Israel.

Cell
|May 29, 1992
PubMed
Summary
This summary is machine-generated.

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Researchers identified the c-abl protein as a key DNA-binding factor within viral enhancers. This protein specifically binds to the EP element, a common functional component found in various viral enhancers.

Area of Science:

  • Molecular biology
  • Virology
  • Oncology

Background:

  • Viral enhancers contain common functional elements like EP that bind cellular proteins.
  • A 140 kDa polypeptide was previously identified as a major EP DNA-binding protein.

Purpose of the Study:

  • To identify the specific cellular protein that binds to the EP element in viral enhancers.
  • To investigate the DNA-binding properties of the identified protein.

Main Methods:

  • Gel retardation analysis to detect protein-DNA complex formation.
  • Ultraviolet cross-linking and Southwestern analysis to identify the major DNA-binding protein.
  • Combination of DNA binding assays and immunological techniques to identify c-abl.

Main Results:

Related Experiment Videos

  • The c-abl protein was identified within a nuclear complex that binds to the EP element.
  • The abl protein exhibits specific and high-affinity DNA binding activity.
  • The mutant abl protein (p210bcr-abl) lacks DNA binding ability, correlating with its cytoplasmic localization in chronic myelogenous leukemia.

Conclusions:

  • The c-abl protein is a significant cellular factor involved in binding to the EP element in viral enhancers.
  • The DNA-binding capacity of abl is crucial for its function, and its absence in p210bcr-abl is linked to disease pathology.