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Updated: May 4, 2026

In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts
Published on: September 2, 2008
Nucleoplasmin remodels sperm chromatin in Xenopus egg extracts
1Wellcome/CRC Institute of Cancer and Developmental Biology, University of Cambridge, England.
Abstract:
Nucleoplasmin is necessary and sufficient for the initial stage of Xenopus sperm decondensation in egg extracts. In this article we show that sperm decondensation is accompanied by loss of two sperm-specific basic proteins (X and Y) and gain of histones H2A and H2B, resulting in nucleosome formation. Purified nucleoplasmin alone removes X and Y and assembles purified H2A and H2B on decondensing sperm chromatin, forming nucleosome cores. Immunodepletion of nucleoplasmin from extract prevents removal of X and Y and addition of H2A and H2B, while adding back nucleoplasmin restores decondensation and X and Y removal. Thus, nucleoplasmin acts as both an assembly and a disassembly factor for remodeling sperm chromatin at fertilization.
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