Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA-coated microcrystals.

Michaela Kreiner1, Geeta Fuglevand, Barry D Moore

  • 1Department of Chemistry, University of Glasgow, Glasgow G12 8Q, UK.

Chemical Communications (Cambridge, England)
|May 27, 2005
PubMed
Summary

Bioactive DNA self-assembles onto crystals like salt and sugar using coprecipitation. This method offers a fast, affordable way to create stable, dry nucleic acid powders for storage, imaging, and drug delivery applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessment of biophysical properties of the first-in-class anti-cancer IgE antibody drug MOv18 IgE demonstrates monomeric purity and stability.

mAbs·2025
Same author

Self-Adjuvanting Calcium-Phosphate-Coated Microcrystal-Based Vaccines Induce Pyroptosis in Human and Livestock Immune Cells.

Vaccines·2023
Same author

Predictors of Survival after Vaccination in a Pneumonic Plague Model.

Vaccines·2022
Same author

Effect of mixing, concentration and temperature on the formation of mesostructured solutions and their role in the nucleation of DL-valine crystals.

Faraday discussions·2015
Same author

Population and size distribution of solute-rich mesospecies within mesostructured aqueous amino acid solutions.

Faraday discussions·2014
Same author

Biophysical characterisation of thermal-induced precipitates of recombinant anthrax protective antigen: evidence for kinetically trapped unfolding domains in solid-state.

European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V·2012

Area of Science:

  • Biochemistry
  • Materials Science
  • Molecular Biology

Background:

  • Nucleic acids require stable formulations for practical applications.
  • Current methods for nucleic acid immobilization can be expensive or complex.
  • Developing cost-effective and rapid methods for nucleic acid stabilization is crucial.

Purpose of the Study:

  • To investigate coprecipitation as a method for immobilizing bioactive DNA.
  • To explore the self-assembly of DNA onto crystalline substrates.
  • To assess the utility of this method for creating dry-powder nucleic acid formulations.

Main Methods:

  • Utilized coprecipitation to induce DNA self-assembly.
  • Employed salt, sugar, and amino-acid crystals as substrates.
  • Characterized the resulting DNA-crystal complexes.

Main Results:

  • Demonstrated successful self-assembly of bioactive DNA onto crystal surfaces.
  • Established coprecipitation as a rapid and inexpensive immobilization technique.
  • Confirmed the suitability of the method for preparing dry-powder nucleic acid formulations.

Conclusions:

  • Coprecipitation provides an efficient strategy for DNA immobilization and formulation.
  • The resulting dry-powder nucleic acid preparations are suitable for storage, imaging, and drug delivery.
  • This technique offers a scalable and accessible approach for nucleic acid stabilization.

Related Experiment Videos