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Updated: Aug 17, 2026

Finite Element Modelling of a Cellular Electric Microenvironment
Published on: May 18, 2021
Retention of nativelike conformation by proteins embedded in high external electric fields
P P Pompa1, A Bramanti, G Maruccio
1National Nanotechnology Laboratories of INFM, University of Lecce, Via per Arnesano 73100 Lecce, Italy.
Abstract:
In this Communication, we show that proteins embedded in high external electric fields are capable of retaining a nativelike fold pattern. We have tested the metalloprotein azurin, immobilized onto SiO2 substrates in air with proper electrode configuration, by applying static fields up to 10(6)-10(7) Vm. The effects on the conformational properties of protein molecules have been determined by means of intrinsic fluorescence measurements. Experimental results indicate that no significant field-induced conformational alteration occurs. Such results are also discussed and supported by theoretical predictions of the inner protein fields.
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