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Updated: Aug 17, 2026

Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS
Published on: November 8, 2015
Cytokine polymorphisms do not influence acute rejection in renal transplantation under tacrolimus-based
M Loucaidou1, J Stitchbury, J Lee
1Renal and Transplant Unit, St Mary's Hospital, London, United Kingdom. mloucaidou@doctors.net.uk
Introduction:
Acute rejection remains an important cause of graft loss after renal transplantation. It has been suggested that cytokine genotyping may play a predictive role in identifying individuals who are at higher risk of acute rejection with a view to individualizing their immunosuppression. The aim of this study was to investigate any possible associations between acute rejection and certain cytokine polymorphisms.
Methods:
We genotyped 91 cadaveric renal transplant recipients on tacrolimus-based immunosuppression and 84 of their donors. The cytokine polymorphisms studied were the following: tumor necrosis factor (TNF)-alpha-1032 T/C, TNF-alpha-865 C/A, TNF-alpha-859 G/A, interleukin (IL)1-R1-970 C/T, IL-10 haplotype [-1082, -819, -592], and IL-6-174 C/G.
Results:
We found no association between any polymorphism and the incidence of acute rejection. This was true for both the recipient and donor population.
Conclusion:
Cytokine polymorphisms did not influence acute rejection in our study. We conclude that in the modern era of immunosuppression cytokine genotyping is not a significant predictor of acute rejection in renal transplantation.
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