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Related Experiment Videos

UV-A induces two calcium waves in Physcomitrella patens.

Edward B Tucker1, Michelle Lee, Shaan Alli

  • 1Natural Science Department, Baruch College, City University of New York, 17 Lexington Avenue, New York, NY 10010, USA. edward_tucker@baruch.cuny.edu

Plant & Cell Physiology
|May 28, 2005
PubMed
Summary

Blue/UV-A light triggers calcium waves in Physcomitrella patens, with distinct patterns observed in cryptochrome and phototropin knock-outs. These calcium signals are crucial for plant responses to light stimuli.

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Area of Science:

  • Plant biology
  • Cell signaling
  • Photoreception

Background:

  • Calcium ions (Ca2+) play a critical role in cellular signaling pathways.
  • Blue/UV-A light photoreceptors, such as cryptochromes and phototropins, are essential for plant responses to light.
  • Understanding Ca2+ dynamics in response to blue/UV-A light is vital for deciphering plant photobiology.

Purpose of the Study:

  • To investigate the role of Ca2+ in blue/UV-A photoreceptor signaling within single cells of Physcomitrella patens.
  • To characterize the spatio-temporal dynamics of intracellular Ca2+ waves induced by UV-A light in wild-type and mutant Physcomitrella patens.

Main Methods:

  • Utilized Fura-2-dextran ratio imaging to visualize Ca2+ dynamics in Physcomitrella patens caulonemal apical cells.
  • Exposed wild-type (WT), Ppcry1a/1b (cryptochrome knock-out), and PpphotA2/B1/B2 (phototropin knock-out) to UV-A radiation.

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  • Analyzed the magnitude, rate, and duration of Ca2+ waves and subsequent Ca2+ spikes.

    • Main Results:

    • UV-A exposure induced two distinct intracellular Ca2+ waves: one originating from the apical tip and another from the neighboring cell junction.
    • Ca2+ wave characteristics (amplitude, rate, duration) differed between WT, Ppcry1a/1b, and PpphotA2/B1/B2 mutants.
    • Subsequent Ca2+ spikes were observed in WT and Ppcry1a/1b but not in PpphotA2/B1/B2, suggesting differential roles of photoreceptors.
    • Manganese (Mn2+) addition altered Ca2+ dynamics, delaying and prolonging the response without wave propagation.

    Conclusions:

    • Plants generate Ca2+ waves in response to blue/UV-A light, indicating a conserved signaling mechanism.
    • The characteristics of these Ca2+ waves are modulated by cryptochrome and phototropin photoreceptors.
    • Blue/UV-A signaling pathways involving Ca2+ in Physcomitrella patens exhibit distinct features compared to those in Arabidopsis.