Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

SECISDesign: a server to design SECIS-elements within the coding sequence.

Anke Busch1, Sebastian Will, Rolf Backofen

  • 1Friedrich-Schiller-University Jena, Institute of Computer Science, Ernst-Abbe-Platz 2, 07743 Jena, Germany.

Bioinformatics (Oxford, England)
|May 28, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhanced IGFL1 translation in response to IL-1β is controlled by distinct 3'UTR elements.

PloS one·2026
Same author

Coordinated alternative splicing decisions via stepwise exon definition.

Nucleic acids research·2026
Same author

Autoencoder/RandomForest-TabPFN for cross-cancer metabolomics: prostate and breast cancer diagnosis using paper spray and ion mobility-mass spectrometry techniques.

GigaScience·2026
Same author

Spark: sparse hierarchical energy minimization for scalable prediction of RNA pseudoknots.

Bioinformatics (Oxford, England)·2026
Same author

A leader-repeat hairpin blocks extraneous CRISPR RNA production in diverse CRISPR-Cas13 systems.

The EMBO journal·2026
Same author

Dissecting the RNA-binding capacity of the multi-RRM protein Rrm4 essential for endosomal mRNA transport.

Nucleic acids research·2026
Same journal

conMItion: an R package adjusting confounding factors for associations in multi-omics.

Bioinformatics (Oxford, England)·2026
Same journal

SpaMFG: a Spatial Multi-omics Integration Method based on Feature Grouping.

Bioinformatics (Oxford, England)·2026
Same journal

CSCN: Inference of Cell-Specific Causal Networks Using Single-Cell RNA-Seq Data.

Bioinformatics (Oxford, England)·2026
Same journal

Sparse CCA-Based Mediation Analysis with High-Dimensional Exposures and Mediators.

Bioinformatics (Oxford, England)·2026
Same journal

Enhancing Cross-Context Generalization in Drug Perturbation Prediction with a Multimodal Conditional Diffusion Framework.

Bioinformatics (Oxford, England)·2026
Same journal

Primer Design through Submodular Function Estimation.

Bioinformatics (Oxford, England)·2026
See all related articles

SECISDesign is a new server for designing RNA elements within mRNA coding sequences. This tool aids in the recombinant expression of selenoproteins by maintaining structural and sequence constraints.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Bioinformatics

Background:

  • Designing RNA elements with specific structural and sequence constraints is crucial for various biological applications.
  • Selenoproteins play vital roles in cellular processes, and their recombinant expression requires precise control over RNA elements.

Purpose of the Study:

  • To develop a computational tool, SECISDesign, for the rational design of RNA elements.
  • To facilitate the creation of SECIS (Selenocysteine Insertion Sequence) elements and other arbitrary RNA structures within mRNA coding sequences.
  • To enable the recombinant expression of selenoproteins in Escherichia coli.

Main Methods:

  • The SECISDesign server integrates algorithms to satisfy both RNA structure and sequence constraints.
  • It ensures a degree of amino acid similarity to the original protein sequence is maintained during the design process.

Related Experiment Videos

  • The server provides a user-friendly interface for designing RNA elements for specific applications.

    • Main Results:

    • SECISDesign successfully generates RNA elements that meet predefined structural and sequence requirements.
    • The designed elements can be effectively utilized for the recombinant expression of selenoproteins.
    • The tool allows for the incorporation of specific RNA structures within the coding sequence of mRNA.

    Conclusions:

    • SECISDesign is a valuable server for researchers involved in RNA design and protein expression.
    • It simplifies the complex process of designing functional RNA elements for selenoprotein synthesis.
    • The server supports the advancement of synthetic biology and protein engineering applications.