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Transcriptome analysis in blastocyst hatching by cDNA microarray.

Huei-Wen Chen1, Jeremy J W Chen, Sung-Liang Yu

  • 1Institute and Department of Pharmacology, School of Medicine, National Yang-Ming University, Taipei, Taiwan.

Human Reproduction (Oxford, England)
|May 28, 2005
PubMed
Summary
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Researchers identified 85 hatching-specific genes in mouse embryos using cDNA microarrays. These genes, including cell adhesion molecules and epigenetic regulators, are crucial for embryo development and may offer new fertility control targets.

Area of Science:

  • Reproductive Biology
  • Developmental Biology
  • Genomics

Background:

  • Blastocyst hatching is vital for embryo development, differentiation, and implantation.
  • Regulatory mechanisms of hatching remain largely unknown.
  • Gene expression profiling offers insights into critical developmental stages.

Purpose of the Study:

  • To identify genes specifically expressed during mouse blastocyst hatching.
  • To investigate the role of these genes in embryo development and implantation.
  • To explore potential therapeutic targets for fertility control.

Main Methods:

  • Collected pre-hatched and hatched ICR mouse embryos for RNA extraction.
  • Utilized RNA amplification and cDNA microarray analysis (6144 genes).

Related Experiment Videos

  • Verified candidate genes via re-sequencing and real-time quantitative RT-PCR.

    • Main Results:

    • Identified 85 genes upregulated in hatched blastocysts compared to pre-hatched ones.
    • Confirmed 47 hatching-related genes, including cell adhesion molecules (E-cadherin, NCAM) and epigenetic regulators (Dnmt1).
    • Observed lower expression of these genes in delayed-growth embryos; confirmed E-cadherin and NCAM localization.

    Conclusions:

    • This study provides key insights into blastocyst hatching mechanisms.
    • Identified hatching-specific genes crucial for implantation.
    • These genes represent potential novel drug targets for fertility regulation.