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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

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Related Experiment Video

Updated: Jul 14, 2026

Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.
11:33

Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.

Published on: May 7, 2009

MicroRNA expression in zebrafish embryonic development.

Erno Wienholds1, Wigard P Kloosterman, Eric Miska

  • 1Hubrecht Laboratory, Centre for Biomedical Genetics, 3584 CT Utrecht, the Netherlands.

Science (New York, N.Y.)
|May 28, 2005
PubMed
Summary

MicroRNAs (miRNAs) regulate gene expression and are crucial for embryonic development. In zebrafish, most miRNAs are tissue-specific later in development, suggesting roles in differentiation rather than initial tissue formation.

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Related Experiment Videos

Last Updated: Jul 14, 2026

Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.
11:33

Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.

Published on: May 7, 2009

In ovo Expression of MicroRNA in Ventral Chick Midbrain
09:19

In ovo Expression of MicroRNA in Ventral Chick Midbrain

Published on: September 16, 2013

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos
08:37

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos

Published on: October 9, 2020

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs (miRNAs) are small noncoding RNAs regulating gene expression.
  • miRNA-mediated gene regulation is vital for embryonic development.
  • Understanding miRNA expression patterns is key to deciphering their developmental roles.

Purpose of the Study:

  • To investigate the temporal and spatial expression of conserved vertebrate miRNAs in zebrafish embryos.
  • To elucidate the role of miRNAs in early versus later stages of embryonic development.

Main Methods:

  • Utilized microarrays to profile miRNA expression.
  • Employed in situ hybridization with locked-nucleic acid (LNA) probes for precise localization.
  • Analyzed expression patterns of 115 conserved vertebrate miRNAs.

Main Results:

  • Most miRNAs exhibited highly tissue-specific expression patterns.
  • This tissue specificity was observed during segmentation and later developmental stages.
  • Early developmental stages showed minimal miRNA expression.

Conclusions:

  • miRNA expression is largely restricted to later stages of zebrafish embryogenesis.
  • The findings suggest miRNAs are primarily involved in tissue differentiation or maintenance of identity, not initial tissue fate establishment.
  • This study provides insights into the functional roles of miRNAs during vertebrate development.