Related Experiment Video
Updated: Aug 17, 2026
A Retrograde Implantation Approach for Peritoneal Dialysis Catheter Placement in Mice
Published on: July 20, 2022
Early peritoneal macrophage function after laparoscopic surgery compared with laparotomy in a mouse mode
U Moehrlen1, F Schwoebel, F Schwöbel
1Department of Pediatric Surgery, University Children's Hospital, Steinwiesstrasse 75, Zurich, CH-8032, Switzerland.
Background:
The authors previously demonstrated postoperative preservation of the immune function measured by delayed-type skin reaction and tumor growth after laparoscopic surgery, as compared with laparotomy. For further elucidation of the origin of the demonstrated immune preservation, peritoneal macrophage (PMo) function was investigated 1 h after different surgical procedures.
Methods:
Female NMRI mice were divided into five groups: anesthesia only, abdominal skin incision, laparotomy, peritoneal carbon dioxide (CO2) insufflation, and peritoneal air insufflation. Escherichia Coli phagocytosis, tumor necrosis factor-alpha (TNF-alpha), transforming growth factor-beta1 (TGF-beta1), and interleukin-10 (IL-10) release of isolated PMo were investigated.
Results:
All invasive interventions reduced the PMo phagocytosis by factors of approximately 2 to 4.7, as compared with the sham control group. Spontaneous ex vivo TNF-alpha release was significantly increased whenever the abdominal cavity was exposed to ambient air. The macrophage's ability to release TNF-alpha after E. coli exposure was diminished in the abdominal air exposure groups, as compared with the CO2 insufflation group.
Conclusion:
Reduced phagocytosis 1 h after surgical interventions suggests a contribution of PMo to the altered immune function. When exposed to CO2, PMo show a decreased basal TNF-alpha release. However, PMo also show an increased TNF-alpha release after a second immune stimulation (E. coli), suggesting a greater competency of interaction in an immune defense reaction after CO2 exposure.
Insights
Surgical interventions impair peritoneal macrophage (PMo) phagocytosis. Carbon dioxide (CO2) insufflation, compared to air, enhances PMo immune response to Escherichia coli, suggesting improved defense post-laparoscopy.
Area of Science:
- Immunology
- Surgical Science
- Cellular Biology
Background:
- Previous studies showed laparoscopic surgery preserves immune function compared to laparotomy.
- Investigating the source of immune preservation is crucial for understanding surgical impact.
Purpose of the Study:
- To elucidate the role of peritoneal macrophages (PMo) in immune preservation after different surgical procedures.
- To compare the effects of CO2 and air insufflation on PMo function.
Main Methods:
- Female NMRI mice underwent anesthesia, skin incision, laparotomy, or CO2/air insufflation.
- Investigated Escherichia coli phagocytosis and release of TNF-alpha, TGF-beta1, and IL-10 from isolated PMo.
Main Results:
- All invasive procedures reduced PMo phagocytosis.
- Abdominal air exposure increased spontaneous TNF-alpha release but diminished E. coli-induced release.
- CO2 insufflation led to decreased basal TNF-alpha but enhanced response to E. coli.
Conclusions:
- Reduced PMo phagocytosis contributes to altered immune function post-surgery.
- CO2 insufflation may enhance PMo's immune defense competency despite reduced basal TNF-alpha release.
More Related Videos
08:21Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation
Published on: April 29, 2015
06:45A Mouse Model of the Associating Liver Partition and Portal Vein Ligation for Staged Hepatectomy Procedure Aided by Microscopy
Published on: January 19, 2024