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[Experimental study on biomechanics characteristics of combined collagen tissue engineering tendon]
1Department of Orthopaedics, West China Hospital, Sichuan University, Chengdu Sichuan, 610041, PR China.
Objective:
To investigate the influence of collagen on the biomechanics strength of tissue engineering tendon.
Methods:
All of 75 nude mice were made the defect models of achilles tendons, and were divided into 5 groups randomly. Five different materials including human hair, carbon fibre (CF), polyglycolic acid (PGA), human hair and PGA, and CF and PGA with exogenous collagen were co-cultured with exogenous tendon cells to construct the tissue engineering tendons. These tendons were implanted to repair defect of achilles tendons of right hind limb in nude mice as experimental groups, while the materials without collagen were implanted to repair the contralateral achilles tendons as control groups. In the 2nd, 4th, 6th, 8th and 12th weeks after implantation, the biomechanical characteristics of the tissue engineering tendon was measured, meanwhile, the changes of the biomechanics strength were observed and compared.
Results:
From the 2nd week to the 4th week after implantation, the experimental groups were stronger than the control groups in biomechanics, there was statistically significant difference (P < 0.05). From the 6th to 12th weeks, there was no statistically significant difference between the experiment and control groups (P > 0.05). Positive correlation existed between time and intensity, there was statistically significant difference (P < 0.05). The strength of materials was good in human hair, followed by CF, and PGA was poor.
Conclusion:
Exogenous collagen can enhance the mechanics strength of tissue engineering tendon, and is of a certain effect on affected limb
