Identification and characterization of a two-component hemolysin from Edwardsiella ictaluri

The channel catfish pathogen Edwardsiella ictaluri possesses hemolysin activity, and strains that are adapted for growth in fish tend to have greater hemolysin activity than strains that are adapted for in vitro growth conditions. To investigate its potential role in virulence, an isogenic hemolysin mutant strain of E. ictaluri R4383 was constructed by transposon mutagenesis. Sequencing of the chromosomal insertion site identified two genes, designated eihA and eihB, that encode proteins with homology to the Serratia family of two-component hemolysins. EihB is similar to the secretion/activation proteins from this family, and EihA is similar to the cytolysin proteins from this family. Bacterial challenge in channel catfish fingerlings did not show a significant difference in virulence between the wild type E. ictaluri strain and the hemolysin deficient E. ictaluri mutant strain.