Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA overstretching transition: ionic strength effects.

Olli Punkkinen1, Per Lyngs Hansen, Ling Miao

  • 1Laboratory of Physics and Helsinki Institute of Physics, Helsinki University of Technology, Finland.

Biophysical Journal
|June 1, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inactive β1-integrin acts as a junctional scaffold for angiopoietin/TIE2/FOXO1 signaling.

The Journal of clinical investigation·2026
Same author

Adhesion-derived condensates control component availability to regulate adhesion dynamics.

Nature communications·2026
Same author

How receptor conformation depends on lipid nanodisc size: Adenosine A<sub>2A</sub> receptor and implications for class-A GPCR proteins.

Biochimica et biophysica acta. Biomembranes·2026
Same author

Structural rewiring of IL-7R dimerization by an oncogenic transmembrane mutation can be reversed by rational design.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Multi-electron nitrobenzothiadiazole sp-conjugated-alkynyl covalent organic frameworks for ammonium-ion batteries.

Nature communications·2026
Same author

HelixSide: A Comprehensive Method for Local and Global Orientational Analysis of Proteins.

Journal of chemical information and modeling·2026

We developed a new model to explain DNA overstretching transitions, finding that salt concentration significantly impacts DNA elasticity. This research offers insights into the forces governing DNA structural changes.

Area of Science:

  • Biophysics
  • Polymer Physics
  • Molecular Biology

Background:

  • Double-stranded DNA (dsDNA) exhibits complex elastic behavior under stretching.
  • Polymer elasticity models describe force increases up to contour length.
  • A highly cooperative overstretching transition occurs beyond contour length.

Purpose of the Study:

  • To develop a theoretical description for the dsDNA overstretching transition.
  • To incorporate monovalent salt effects on elastic moduli into the model.
  • To gain insight into the mechanisms governing force-induced DNA overstretching.

Main Methods:

  • Coupling a two-state model with existing elasticity theory.
  • Extending the model to include salt concentration effects.

Related Experiment Videos

  • Comparing theoretical predictions with experimental measurements of dsDNA overstretching.

    • Main Results:

    • The theoretical model accurately predicts experimental salt dependence of the overstretching transition.
    • Effective DNA length per charge varies with salt, consistent with polyelectrolyte models.
    • Structural parameters show minimal salt dependence, indicating elasticity mediates overstretching.

    Conclusions:

    • The developed model provides a robust framework for understanding DNA overstretching.
    • Electrostatic forces influencing overstretching are mediated by mesoscopic elasticity.
    • Salt effects are crucial for accurately modeling DNA mechanical properties.