Precision and variance components in quantitative gel electrophoresis
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Updated: May 2, 2026

A Guide to Modern Quantitative Fluorescent Western Blotting with Troubleshooting Strategies
Published on: November 21, 2014
Angela Koller1, Hermann Wätzig
1Roche Diagnostics GmbH, Analytical Support, Mannheim, Germany.
Abstract:
The error in quantitative gel electrophoresis/Western blotting was investigated considering the purity testing of erythropoietin. The overall error was over 35% relative standard deviation. However, an analysis of variance elucidated that the interoperator variability was the dominant error source, which already explained almost 80% of the total variance. Careful compilation and investigation of the possible error sources strongly indicates that the immunoreaction after blotting and the subsequent color reaction are the major error sources in this case.
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