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Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation
06:47

Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation

Published on: January 28, 2021

[Polymorphisms in the apolipoprotein A5 gene and apolipoprotein C3 gene in patients with coronary artery disease]

Nan Bi1, Sheng-Kai Yan, Guo-Ping Li

  • 1Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China.

Zhonghua Xin Xue Guan Bing Za Zhi
|June 1, 2005
PubMed

Insights

The APOA5 -1131T/C gene variant is linked to a higher risk of coronary artery disease (CAD) in the Chinese Han population, associated with elevated triglycerides. The APOC3 -482C/T variant showed no such association with CAD risk.

Area of Science:

  • Genetics
  • Cardiovascular Disease
  • Molecular Biology

Background:

  • Coronary artery disease (CAD) is a leading cause of mortality worldwide.
  • Genetic factors, including polymorphisms in apolipoprotein genes, play a role in CAD susceptibility.
  • APOA5 and APOC3 genes are key regulators of triglyceride metabolism and have been implicated in cardiovascular risk.

Purpose of the Study:

  • To investigate the association between specific polymorphisms in the APOA5 gene (-1131T/C, 56C/G) and the APOC3 gene (-482C/T) with coronary artery disease (CAD) risk.
  • To determine if these genetic variations correlate with serum lipid profiles in a Chinese Han population.

Main Methods:

  • Genotyping of APOA5 (-1131T/C, 56C/G) and APOC3 (-482C/T) polymorphisms using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and polyacrylamide gel electrophoresis (PAGE).
  • Analysis of genotype frequencies in 312 CAD patients and 317 healthy controls.
  • Biochemical analysis of serum lipid profiles.

Main Results:

  • The APOA5 -1131 C allele was significantly more frequent in CAD patients (39.9%) compared to controls (33.3%, P=0.02).
  • APOA5 -1131 CC homozygotes exhibited a significantly increased risk of CAD (OR=1.93 unadjusted, OR=1.80 adjusted).
  • The APOA5 -1131C allele correlated with elevated plasma triglyceride (TG) levels (P<0.01).

Conclusions:

  • The APOA5 -1131T/C polymorphism is associated with an increased risk of CAD in the Chinese population.
  • This association is linked to elevated serum TG levels and appears independent of the APOC3 -482C/T variant.
  • The APOC3 -482C/T polymorphism did not show a significant association with CAD risk in this cohort.
Abstract

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