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Related Experiment Videos

Peak capacity in gradient ultra performance liquid chromatography (UPLC).

Stephen A C Wren1

  • 1Analytical development, PAR&D, AstraZeneca, Silk Business Park, Macclesfield, Cheshire, SK10 2NA, UK. Stephen.wren@astrazeneca.com

Journal of Pharmaceutical and Biomedical Analysis
|June 1, 2005
PubMed
Summary

Ultra-performance liquid chromatography (UPLC) using short columns at high flow rates maximizes peak capacity for fast analyses. Longer analyses may benefit from longer columns for increased peak capacity.

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Area of Science:

  • Analytical Chemistry
  • Chromatography

Background:

  • Ultra-performance liquid chromatography (UPLC) offers enhanced separation efficiency.
  • Optimizing peak capacity is crucial for complex mixture analysis in pharmaceutical development.

Purpose of the Study:

  • To investigate peak capacity in UPLC using a prototype commercial instrument.
  • To determine the impact of gradient time, flow rate, and column length on peak capacity for small molecule pharmaceuticals.

Main Methods:

  • Utilized a prototype commercial UPLC instrument with 2.1 mm i.d. columns packed with 1.7 µm porous particles.
  • Measured peak capacity for a small molecule pharmaceutical under varying analytical conditions.

Main Results:

  • Short columns operating at high linear velocities yield the highest peak capacity for rapid UPLC analyses.

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  • Increasing analysis time necessitates longer columns to achieve further gains in peak capacity.

    • Conclusions:

    • Analytical method development in UPLC requires balancing speed and resolution.
    • Column length and analysis time are key parameters for optimizing peak capacity in UPLC separations.