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Related Experiment Videos

Plant transposable elements generate the DNA sequence diversity needed in evolution.

Z Schwarz-Sommer1, A Gierl, H Cuypers

  • 1Max-Planck-Institut für Züchtungsforschung, 5000 Köln 30, FRG.

The EMBO Journal
|March 1, 1985
PubMed
Summary
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Transposable elements in maize, like the Enhancer (En) system, can cause mutations by excising from genes. This study analyzed DNA changes, revealing how these elements drive molecular evolution and create new gene functions.

Area of Science:

  • Molecular Biology
  • Genetics
  • Plant Science

Background:

  • Transposable elements (TEs) are mobile genetic sequences that can alter genome structure and function.
  • The Enhancer (En) transposable element system in maize is a well-studied model for TE activity.
  • Understanding TE-mediated mutations is crucial for studying genome evolution and gene function.

Purpose of the Study:

  • To clone and sequence reversion events induced by the Spm-I8 element in Zea mays.
  • To analyze the DNA alterations resulting from Spm-I8 excision from the wx gene.
  • To investigate the role of TE activity in generating sequence divergence and potentially new gene functions.

Main Methods:

  • Cloning of revertant alleles.
  • DNA sequence analysis of wild-type and mutant wx alleles.

Related Experiment Videos

  • Comparison of intron sequences to identify potential 'foot-print' sequences.

    • Main Results:

    • Characterization of two germinal and 16 somatic reversion events.
    • Identification of various mutant DNA sequences resulting from Spm-I8 excision.
    • Observation of potential 'foot-print' sequences in intron regions.

    Conclusions:

    • Spm-I8 excision leads to diverse mutations in the wx gene.
    • TE activity contributes to sequence divergence at the molecular level.
    • TE-induced changes may play a role in the evolution of novel gene functions.