Related Experiment Video
Updated: Aug 9, 2026
Analysis of Gene Expression in Emerald Ash Borer (Agrilus planipennis) Using Quantitative Real Time-PCR
Published on: May 4, 2010
Developmental expression patterns of four chemosensory protein genes from the Eastern spruce budworm, Chroistoneura
K W Wanner1, M B Isman, Q Feng
1Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, Summerland, British Columbia, Canada.
Abstract:
Chemosensory proteins (CSPs) are associated with insect sensory organs, including the sensillum lymph in some cases. However, they are also commonly expressed in nonsensory tissues that lack gustatory and olfactory neurones. We characterized the sex and development specific expression patterns of four CSP genes from the Eastern spruce budworm (ESB) using Northern blots. CfumAY426540.2 was detected at high levels in adult moths. Conversely, CfumAY426538 was expressed in all stages except adult moths, and was most abundant during late stages of the 6th instar. CfumAY701858 was expressed in all stages, while CfumAY426539 was detected less frequently, at specific developmental stages such as the 5th to 6th instar moult. During a natural moult, and a premature moult induced by the ecdysteroid agonist tebufenozide, CfumAY701858 and CfumAY426539 were up-regulated, while CfumAY426538 appeared to be down-regulated. Our results suggest that some members of the CSP gene family from the ESB may be involved in development, including moulting.
