Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Protein sorting in the Golgi complex: shifting paradigms.

Enrique Rodriguez-Boulan1, Anne Müsch

  • 1Margaret Dyson Vision Research Institute, Weill Medical College of Cornell University, LC-300, 1300 York Avenue, New York, NY 10021, USA. boulan@med.cornell.edu

Biochimica Et Biophysica Acta
|June 2, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bile canaliculi formation in primary hepatocytes requires α1β1 integrin-dependent adherens junction re-organization.

Journal of cell science·2025
Same author

Stromal-Like Cells and Retinal Pigment Epithelium Modulate Choroidal Sprouting Through Galectin-1-Dependent and Independent Pathways.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2025
Same author

Borg5 restricts contractility and motility in epithelial MDCK cells.

Journal of cell science·2024
Same author

Regulation of the apico-basolateral trafficking polarity of the homologous copper-ATPases ATP7A and ATP7B.

Journal of cell science·2023
Same author

Retinal Pigment Epithelium-Secreted VEGF-A Induces Alpha-2-Macroglobulin Expression in Endothelial Cells.

Cells·2022
Same author

Live-cell Imaging of Biosynthetic Protein Transport in Hepatocytes.

Methods in molecular biology (Clifton, N.J.)·2022

Cellular transport has evolved, with sorting signals now crucial at multiple pathway steps. Key adaptors like AP1B and AP4 mediate protein sorting, particularly from the Golgi complex.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • The understanding of intracellular transport has significantly advanced in the last 15 years.
  • Previously, protein sorting was thought to occur primarily in the Golgi complex.

Purpose of the Study:

  • To outline the current paradigm of protein transport and sorting along the biosynthetic pathway.
  • To highlight the critical role of sorting signals at various stages of intracellular transport.

Main Methods:

  • Review of current literature on protein trafficking and sorting mechanisms.
  • Analysis of the roles of specific adaptor proteins in protein targeting.

Main Results:

  • The current model emphasizes sorting signals at nearly every step of the biosynthetic pathway.

Related Experiment Videos

  • Protein sorting occurs not only in the Golgi complex and Trans Golgi Network (TGN) but also distally (recycling endosomes) and proximally (ER exit).
  • Adaptor proteins AP1B and AP4 are identified as key players in apical and basolateral protein sorting.

    • Conclusions:

    • Intracellular protein sorting is a complex, multi-step process involving signals throughout the biosynthetic route.
    • The localization of sorting events extends beyond the Golgi, involving earlier and later cellular compartments.
    • Ongoing research is expected to further clarify the mechanisms and adaptors involved in protein trafficking.