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Inp1p is a peroxisomal membrane protein required for peroxisome inheritance in Saccharomyces cerevisiae

Monica Fagarasanu1, Andrei Fagarasanu, Yuen Yi C Tam

  • 1Department of Cell Biology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada.

The Journal of Cell Biology
|June 2, 2005
PubMed

Insights

Inp1p is crucial for peroxisome inheritance in yeast cells. This protein retains peroxisomes within the mother cell and influences their division, representing the first such factor identified for peroxisomes.

Area of Science:

  • Cell Biology
  • Organelle Inheritance
  • Yeast Genetics

Background:

  • Cells possess mechanisms for organelle transmission during division.
  • Peroxisome inheritance mechanisms remain poorly understood.
  • Inp1p, a peripheral membrane protein, influences peroxisome morphology and partitioning.

Purpose of the Study:

  • To investigate the role of Inp1p in peroxisome inheritance and cell division.
  • To elucidate the molecular mechanisms underlying peroxisome partitioning.

Main Methods:

  • In vivo 4-dimensional video microscopy.
  • Analysis of yeast cells lacking or overexpressing the INP1 gene.
  • Protein localization studies.
  • Co-immunoprecipitation assays.

Main Results:

  • Cells lacking INP1 showed impaired peroxisome retention in the mother cell.
  • Overexpression of INP1 led to immobilized peroxisomes, preventing bud partitioning.
  • Inp1p localizes to peroxisomes and the cell cortex, suggesting interactions with cellular structures.
  • Inp1p interacts with Pex25p, Pex30p, and Vps1p, proteins involved in peroxisome division.

Conclusions:

  • Inp1p functions as a retention factor for peroxisomes within the mother cell.
  • Inp1p plays a role in regulating peroxisome division.
  • Inp1p is the first identified peroxisomal protein directly involved in peroxisome inheritance.

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