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Related Experiment Videos

Group B streptococcal opacity variants.

S H Pincus1, R L Cole, M R Wessels

  • 1Laboratory of Microbial Structure, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840.

Journal of Bacteriology
|June 11, 1992
PubMed
Summary
This summary is machine-generated.

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Group B Streptococcus (GBS) exhibits colony opacity variants. Opaque variants show altered cell surface properties and aggregation, impacting GBS characteristics and potential pathogenesis.

Area of Science:

  • Microbiology
  • Bacterial genetics
  • Pathogenesis

Background:

  • Group B Streptococcus (GBS) is a significant human pathogen.
  • Colony morphology variations, such as opacity, can influence bacterial behavior.

Purpose of the Study:

  • To investigate the characteristics of different colony opacity variants of type III GBS.
  • To explore the relationship between colony opacity and bacterial cell surface properties, aggregation, and potential virulence factors.

Main Methods:

  • Culturing and characterization of GBS opacity variants on various media.
  • Scanning and transmission electron microscopy to assess bacterial aggregation.
  • Analysis of surface proteins, capsular polysaccharide content, and electrophoretic mobility.

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Main Results:

  • Two stable opaque variants (1.1 and 1.5) and three transparent clones were characterized.
  • Opaque variants exhibited increased bacterial aggregation and chain formation.
  • Variant 1.1 showed unique surface proteins and deficient type III polysaccharide, while variant 1.5 lacked group B antigen.
  • Opacity switching occurred in vitro and in vivo, though variant 1.5 was stable.

Conclusions:

  • GBS exists in both opaque and transparent forms, arising through multiple mechanisms.
  • Opacity variation is linked to changes in cell surface characteristics and aggregation.
  • The clinical relevance of GBS opacity variation remains undetermined due to altered growth on standard media.