Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microdissection-derived murine mcb probes from somatic cell hybrids.

Vladimir Trifonov1, Constanze Karst, Uwe Claussen

  • 1Institut für Human Genetics and Anthropology, Postfach, D-07740 Jena, Germany.

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
|June 2, 2005
PubMed
Summary

The multicolor-banding (mcb) technique uses region-specific libraries for chromosome analysis. New mcb-probe sets for mouse chromosomes 3, 6, and 18 improve identification, especially for hard-to-distinguish chromosomes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From X-inactivation to neurodevelopment: CHD8-transcription factors (TFs) competitive binding at regulatory regions of CHD8 target genes can contribute to correct neuronal differentiation.

Current research in neurobiology·2023
Same author

Intrachromosomal amplification of BCR expressed as homogenously staining region (hsr) in a case of acute myeloid leukemia with myelodysplasia-related changes.

International journal of laboratory hematology·2022
Same author

Acute myeloid leukemia due to germline CEBPA mutation in a Syrian family.

Molecular genetics & genomic medicine·2022
Same author

Casein Kinase 1D Encodes a Novel Drug Target in Hedgehog-GLI-Driven Cancers and Tumor-Initiating Cells Resistant to SMO Inhibition.

Cancers·2021
Same author

Identification of novel drug resistance mechanisms by genomic and transcriptomic profiling of glioblastoma cells with mutation-activated EGFR.

Life sciences·2021
Same author

Chd8 regulates X chromosome inactivation in mouse through fine-tuning control of Xist expression.

Communications biology·2021

Area of Science:

  • Cytogenetics
  • Molecular Biology
  • Genomics

Background:

  • Standard chromosome banding techniques like GTG-banding can struggle to differentiate between morphologically similar mouse chromosomes.
  • Fluorescence in situ hybridization (FISH) offers advanced cytogenetic analysis, but multicolor-banding (mcb) provides enhanced resolution.
  • Multicolor-banding relies on region-specific microdissection libraries to generate distinct fluorescence intensity ratios along chromosomes.

Purpose of the Study:

  • To introduce the first available multicolor-banding (mcb) probe sets for *Mus musculus* chromosomes 3, 6, and 18.
  • To demonstrate the utility of mcb in improving the identification of mouse chromosomes.
  • To establish a novel method for creating mcb-probe sets using mouse/human somatic cell hybrids.

Main Methods:

Related Experiment Videos

  • Development of region-specific microdissection libraries for *Mus musculus* chromosomes.
  • Creation of multicolor-banding (mcb) probe sets utilizing these libraries.
  • Application of mcb-probe sets on *Mus musculus* chromosomes, with initial library creation performed on mouse/human somatic cell hybrids.

Main Results:

  • Successfully generated and validated mcb-probe sets for mouse chromosomes 3, 6, and 18.
  • The mcb technique provided unambiguous identification of mouse chromosomes, overcoming limitations of GTG-banding.
  • The use of mouse/human somatic cell hybrids proved effective for creating mcb-probe libraries.

Conclusions:

  • The developed mcb-probe sets represent a significant advancement for *Mus musculus* cytogenetic analysis.
  • Multicolor-banding offers superior resolution for distinguishing even closely related murine chromosomes.
  • This work establishes a new protocol for mcb-probe library creation applicable to other species.