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Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
12:15

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Published on: May 29, 2019

[DNA damage induced by products of lipid peroxidation]

Waldemar M Przybyszewski1, Janusz Kasperczyk, Katarzyna Stokłosa

  • 1Zakład Radiobiologii Doświadczalnej i Klinicznej, Centrum Onkologii, Instytut im.M.Skłodowskiej-Curie, Oddział w Gliwicach, 44-101 Gliwice. wmp@io.gliwice.pl

Postepy Higieny I Medycyny Doswiadczalnej (Online)
|June 2, 2005
PubMed

Insights

Lipid peroxidation products form bulky DNA adducts, causing genome instability and promoting cancer. DNA repair pathways like base excision repair (BER) and nucleotide excision repair (NER) help fix these harmful lesions.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Context:

  • Lipid peroxidation generates reactive aldehydes.
  • These aldehydes adduct to DNA, forming bulky lesions.
  • Bulky DNA adducts are implicated in mutagenesis and cancer.

Purpose:

  • To highlight the role of bulky DNA adducts in genome instability.
  • To discuss specific adducts like etheno- and propanoadducts.
  • To review DNA repair mechanisms involved in lesion removal.

Summary:

  • Adduction of aldehydic end-products of lipid peroxidation to DNA creates bulky adducts, leading to genome instability.
  • These bulky-DNA adducts are miscoding and contribute to mutagenesis and cancerogenesis.
  • Etheno- and propanoadducts are key DNA modifiers repaired by base excision repair (BER), nucleotide excision repair (NER), nucleotide incision repair (NIR), and transcription-coupled repair (TCR).

Impact:

  • Understanding these adducts and repair pathways is crucial for cancer prevention and treatment.
  • This knowledge can inform strategies to mitigate DNA damage from oxidative stress.
  • Identifying specific repair mechanisms provides targets for therapeutic intervention.

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