Enhancement of oxygen mass transfer in stirred bioreactors using oxygen-vectors 2. Propionibacterium shermanii broths
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Updated: Aug 17, 2026

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
Published on: December 15, 2017
Anca-Irina Galaction1, Dan Cascaval, Marius Turnea
1Faculty of Medical Bioengineering, Department of Biotechnology, University of Medicine and Pharmacy, University Street 16, 6600 Iasi, Romania. galact@from.ro
Abstract:
The previous works on simulated broths are continued and developed for Propionibacterium shermanii broths. The obtained results indicated the considerable increase of kLa in presence of n-dodecane as oxygen-vector and the existence of a certain value of hydrocarbon concentration that corresponds to the maximum mass transfer rate of oxygen. The magnitude of the positive effect of the oxygen-vector strongly depends on operational conditions of the bioreactor, on broth characteristics and on P. shermanii concentration.
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