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Glial-guided neuronal migration in P19 embryonal carcinoma stem cell aggregates
Marcelo F Santiago1, Sean S Liour, Rosalia Mendez-Otero
1Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. felipe@biof.ufrj.br
Journal of Neuroscience Research
|June 2, 2005
Summary
This study introduces a new P19 cell model to investigate neuronal migration along radial glia. Findings show 9-O-acetyl GD3 ganglioside is crucial for this glial-guided migration.
Area of Science:
- Neuroscience
- Developmental Biology
- Cell Biology
Background:
- Neuronal precursors migrate along radial glial fibers during nervous system development.
- P19 embryonal carcinoma stem cells differentiate into neural and glial lineages upon retinoic acid (RA) exposure.
Purpose of the Study:
- To establish and characterize a novel P19 cell culture model for studying glial-guided neuronal migration.
- To investigate the role of 9-O-acetyl GD3 ganglioside in this migration process.
Main Methods:
- P19 aggregates were induced with RA and cultured on poly-L-lysine coated dishes.
- Cell migration, morphology, and protein expression (GFAP, nestin, MAP2) were analyzed.
- Scanning electron microscopy (SEM) and HPTLC were employed.
- Antibody perturbation assays were used to assess the role of 9-O-acetyl GD3.
Main Results:
- Differentiated P19 cells exhibited migration patterns resembling neuronal precursors on radial glia-like fibers.
- Migrating cells expressed neuronal and glial markers, with intimate association to radial fibers.
- The ganglioside 9-O-acetyl GD3 was expressed and its blockade reversibly inhibited neuronal migration.
Conclusions:
- A new in vitro model using P19 cells effectively mimics glial-guided neuronal migration.
- 9-O-acetyl GD3 plays a significant role in regulating this type of neuronal migration.
