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Published on: April 26, 2016
Non-reductive scavenging of 1,1-diphenyl-2-picrylhydrazyl (DPPH) by peroxyradical: a useful method for quantitative
Masahiro Nishizawa1, Masahiro Kohno, Minemitsu Nishimura
1New Industry Creation Hatchery Center, Tohoku University, Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan.
Abstract:
A stable radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) has long been used as a convenient method for the antioxidant assay of biological materials such as cysteine, glutathione, ascorbic acid, tocopherol and polyhydroxy aromatic compounds (hydroquinone, pyrogallol, etc.). In this study, non-reductive scavenging of DPPH was investigated by electron spin resonance (ESR) analyses for the purpose of developing a useful method for quantitative determination of peroxyradical. Since DPPH was degraded in the presence of peroxyradical derived from UV-irradiated benzoylperoxide and the peroxyradical-induced degradation of DPPH was inhibited by the addition of a spin trapping agent 5,5-dimethyl-1-pyrroline-N-oxide (DMPO), it is concluded that DPPH is non-reductively scavenged by peroxyradical. Therefore, it is suggested that DPPH could be a useful agent for the quantitative measurement of peroxyradical.
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