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Related Experiment Videos

Voltage-clamp errors cause anomalous interaction between independent ion channels.

Hamilton E Farris1, Anthony J Ricci

  • 1Neuroscience Center and Kresge Hearing Labs, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA. hfarri@lsuhsc.edu

Neuroreport
|June 3, 2005
PubMed
Summary

Uncompensated series resistance in voltage-clamp experiments can cause errors by activating unintended channels, especially in hair cells. This error, unlike others, cannot be corrected after data collection.

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Area of Science:

  • Electrophysiology
  • Cell Physiology
  • Neuroscience

Background:

  • Uncompensated series resistance (Rs) in voltage-clamp introduces steady-state voltage errors.
  • These errors typically scale with elicited current amplitude and are often correctable offline.
  • However, Rs-induced errors can become problematic when investigating complex cellular systems.

Purpose of the Study:

  • To investigate the impact of uncompensated series resistance on voltage-clamp recordings of mechanoelectric transduction currents in hair cells.
  • To determine if Rs-induced voltage errors can erroneously activate other voltage-dependent conductances.
  • To highlight the limitations of offline correction for specific electrophysiological artifacts.

Main Methods:

  • Voltage-clamp recordings were performed on hair cells.

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  • Sensory hair bundles were displaced to elicit mechanoelectric transduction currents.
  • The influence of uncompensated series resistance on holding potential and channel activation was analyzed.

    • Main Results:

    • Steady-state voltage errors (<1.5 mV) were induced by uncompensated series resistance at hair cells' resting potential.
    • These errors led to the erroneous activation of voltage-gated calcium channels and calcium-activated potassium channels (BK).
    • The observed Rs-induced voltage errors were not correctable offline.

    Conclusions:

    • Uncompensated series resistance can interact with elicited currents to produce voltage errors that erroneously activate other conductances.
    • This artifact is particularly critical in systems with multiple, steeply voltage-dependent conductances, such as hair cells.
    • Researchers must recognize and account for this non-correctable error when interpreting voltage-clamp data.