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Related Experiment Videos

Programmed cell death and leaf morphogenesis in Monstera obliqua (Araceae).

Arunika H L A N Gunawardena1, Kathy Sault, Petra Donnelly

  • 1Department of Botany, University of Toronto, Toronto, ON, M5S 1A1, Canada.

Planta
|June 3, 2005
PubMed
Summary

Monstera obliqua leaves develop holes via programmed cell death, where cells self-destruct simultaneously. This process, involving DNA fragmentation, creates unique leaf perforations through convergent evolution.

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Area of Science:

  • Plant biology
  • Developmental biology
  • Morphogenesis

Background:

  • Monstera obliqua exhibits unique leaf perforations.
  • Leaf development involves complex cellular processes.

Purpose of the Study:

  • To investigate the cellular mechanisms behind perforation formation in Monstera obliqua.
  • To understand the role of programmed cell death in leaf morphogenesis.

Main Methods:

  • Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay for DNA cleavage.
  • Gel electrophoresis to analyze DNA fragmentation patterns.
  • Ultrastructural analysis using electron microscopy.

Main Results:

  • Programmed cell death occurs simultaneously in discrete cell subpopulations at perforation sites.

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  • DNA cleavage, indicated by TUNEL-positive nuclei, is an early event.
  • Ultrastructural analysis revealed characteristic cellular changes including condensed chromatin and disrupted vacuoles.
  • Cell walls remain intact, and adjacent cells differentiate into epidermal cells.

    • Conclusions:

    • Perforation formation in Monstera obliqua is mediated by simultaneous programmed cell death.
    • The process involves random DNA cleavage without internucleosomal fragmentation.
    • This mode of leaf morphogenesis is a result of convergent evolution, distinct from previously studied cases.