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Updated: Feb 11, 2026

Profiling of Permethylated Mucin O-glycans Using Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry
Published on: June 20, 2025
[Study on identification of aconiti by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry]
1Department of Forensic Toxicological Analysis, Preclinical School, Sun Yat-Sen University, Guangzhou 510089, China.
Objective:
The merhod of determination of traditional Chinese medicine of aconitum root has been established by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
Methods:
With optimized matrix of 2,5-Dihydroxy- benzoic acid for herbal extract, the identification of aconitum root by MALDI-TOF-MS has been conditioned. By comparison of mass spetra of various of aconitium root, their fingerprint mass-to-charge ratios were found to perform identification. With the characteristic mass-to-charge ratios, It was shown that the mass spectrum of raw aconitum roots were obviously different from that of processed aconitum roots.
Results:
The raw radix aconiti could be differentiated from radix aconiti preparate by determination of MALDI-TOF-MS.
Conclusion:
The identifical method of aconitum root by MALDI-TOF-MS is rapid, precise and sensitive, it can be used in toxicological analysis of medical tangle and illegal medical practice.
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