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Updated: Aug 17, 2026

Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model
Published on: September 22, 2020
[Sequences and their polymorphisms of swine MHC-DQB, DRB proximal promoter region]
Ru-Hai X U1, Kun Wang, Dong-Xiao Sun
1College of Animal Science & Technology, China Agricuutural Univeisity, Beijing 100094, China.
Abstract:
The primers, RF/RR and QF1/QR,were designed based on the homologous region of swine MHC-DQB and DRB exon1 sequences and HLA-DQB and HLA-DRB sequences. The 5' proximal promoter region (URR) nucleotide sequences of swine MHC-DQB and DRB were firstly obtained by PCR, cloning and sequencing techniques. There exist highly conserved sequence motifs including W, X, Y, CCAAT, and TATA-like boxes in the obtained sequences,showing the same organization of the conserved regulatory elements as in other species. 12 DRB-URR and 14 DQB-URR alleles were found by PCR-SSCP in 313 pigs. The aligned results of these alleles showed that there exist abundant polymorphism sites in both Porcine MHC-DQB and DRB proximal promoter region.
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