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Updated: Aug 17, 2026
Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
Published on: March 24, 2017
[Preparation and identification of monoclonal antibody against heterogeneous nuclear ribonucleoprotein B1]
Ming-yong Chen1, Wei-min Li, Dan Xu
1Respiratory Department, West China Hospital, Sichuan University, Chengdu, China.
Objective:
To establish anti-heterogeneous nuclear ribonucleoprotein B1 monoclonal antibody cell line and to identify immunological activity.
Methods:
BALB/c mice were immunized with specific hnRNPB1 polypeptide by multiple-place hypodermic injection. The splenic cells of the mice were fused with SP2/0 mouse myeloma cells. The hybridoma culture supernants were screened by indirect ELISA with artificial synthetic peptide.
Results:
Two hybridoma cell lines against hnRNPB1 were successfully obtained; the titers of antibody in ascites were 1: 1.8 x 10(5). The McAb showed the character of no cross reactions. Immunohistochemical analysis demonstrated that the antibody could bind with hnRNPB1 specifically.
Conclusion:
The McAb prepared so by this laboratory is of good specificity and sensitivity.
