Related Experiment Video
Updated: Aug 17, 2026

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
Published on: March 8, 2012
[The inhibitory effects of siRNA expression vector on the expression of human papillomavirus E6 gene]
Da-ke Li1, Zhi-lan Peng, Xiao-yu Niu
1Department of Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To investigate the inhibitory effects of RNAi(RNA interference)expression vector on the expression of human papilomavirus E6 gene.
Methods:
siRNA expression vectors were constructed to be aimed directly at HPV16 E6 gene. The recombinants were transfected into cervical cancer cell line, caski, with liposomes. Expression of E6 was detected with fluorescence quantitative PCR (FQ-PCR) and flow cytometry.
Results:
Three kinds of expression vectors could reduce the expression of E6 mRNA and protein all in caski-B cell. The expression of E6 mRNA reduced to 21.7% of control group, and the protein inhibition ratio reached 98.1%.
Conclusion:
The RNAi expression vector can effectively inhibit the expression of HPVE6 gene.
Related Concept Videos
Inhibitors of Viral Protein Synthesis
Experimental RNAi
Inhibitors of Virion Maturation and Assembly
Inhibitors Of Virion Release
MicroRNAs
RNA Interference
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

