[Effect of blood purification materials and anticoagulants on the contact activity of factor XI]
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Updated: Aug 17, 2026

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
Published on: September 9, 2012
Li-ping Cheng1, Shu-dong Sun, Yi-lun Yue
1Center for Biomedical Engineering, College of Material Science and Engineering, Sichuan Univeisity, Chengdu 610065, China.
Objective:
This study was conducted to examine the degree of contact activation of five blood purification materials sulfonated polyethersulfone (PES-SO3Na), polyethersulfone (PES), polysulfone (PS), polymethylmethacrylate (PMMA) and cellulose acetate (CA) after they were incubated in anticoagulant-containing human plasma for 10 minutes at 37 degrees C.
Methods:
Three anticoagulants Heparin, Trisodium citrate and Disodium EDTA were used. The time course for generation of FXII activity by the materials after incubation in anticoagulant-containing human plasma was evaluated. The degree of contact activation was determined by measuring membrane-bound alpha-FXIIa activity level.
Results:
(1) The five materials caused different increase at different rate in the generation of alpha-FXIIa after they were separately incubated with each of the three anticoagulants. (2) PES-SO3Na activated the least FXII no matter which anticoagulant the plasma contained.
Conclusion:
Contact activation can be mediated by antithrombotic agent. PES-SO3Na has the best blood compatibility with respect to contact activation considering intrinsic coagulation.
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