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Updated: Aug 15, 2026
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Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
Published on: March 30, 2015
Development and evaluation of a broadly reactive TaqMan assay for rapid detection of hepatitis A virus
N Jothikumar1, T L Cromeans, M D Sobsey
1Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, 27599-7431, USA. JIN2@cdc.gov
Applied and Environmental Microbiology
|June 4, 2005
Abstract:
Primers and a TaqMan probe for the 5'-untranslated region (UTR) of the hepatitis A virus (HAV) genome were designed and evaluated. The assay detected 0.5 infectious units of HAV and 40 copies of a synthetic transcript and provides an important screening tool for rapid quantitative HAV detection in clinical or environmental samples.
