Related Experiment Video
Updated: Aug 17, 2026
Production and Purification of Non Replicative Canine Adenovirus Type 2 Derived Vectors
Published on: December 3, 2013
Molecular cloning of the cDNA of canine homeodomain-interacting protein kinase 2
Sook-Yeon Lee1, Jin-Young Chung, Il-Seob Shin
1Department of Veterinary Internal Medicine, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul, Korea.
Abstract:
The research of p53 is being conducted to find the mechanisms of tumorigenesis and to treat various cancers. Homeodomain-interacting protein kinase2 (HIPK2) is an important factor to regulate p53 and to increase the stability of p53. Activation of HIPK2 leads to the selective phosphorylation of p53, resulting in growth arrest and the enhancement of apoptosis. In this study, the canine HIPK2 cDNA fragments were obtained, and their overlapping regions were aligned to give a total sequence of 3489 bp. The canine HIPK2 cDNA (GenBank accession number; AY800385) shares 93% and 90% sequence identity with those of human and mouse HIPK2, respectively. The canine HIPK2 cDNA contains an open reading frame encoding 1163 amino acid residues and the predicted amino acid sequence has 98% and 96% identity with those of human and mouse, respectively. The deduced amino acid sequence of canine HIPK2 has also all domains' sites compared with human and mouse HIPK2. Therefore, these structural similarities suggested that the canine HIPK2 shares the basic biological functions that HIPK2 exhibit in other species.
Insights
Researchers identified canine Homeodomain-interacting protein kinase2 (HIPK2) cDNA, crucial for regulating p53 stability and cancer mechanisms. Canine HIPK2 shares high sequence identity with human and mouse counterparts, suggesting conserved biological functions.
Area of Science:
- Molecular Biology
- Cancer Research
- Genetics
Background:
- p53 research is vital for understanding tumorigenesis and developing cancer treatments.
- Homeodomain-interacting protein kinase2 (HIPK2) plays a key role in regulating p53 stability and function.
- HIPK2 activation leads to p53 phosphorylation, inducing cell growth arrest and apoptosis.
Purpose of the Study:
- To obtain and characterize the canine HIPK2 cDNA sequence.
- To compare canine HIPK2 with its human and mouse orthologs.
- To infer the functional conservation of canine HIPK2.
Main Methods:
- Isolation and sequencing of canine HIPK2 cDNA fragments.
- Alignment of overlapping cDNA fragments to assemble the full sequence.
- Bioinformatic analysis to determine sequence identity and domain conservation.
Main Results:
- A complete canine HIPK2 cDNA sequence of 3489 bp was obtained (GenBank accession number AY800385).
- Canine HIPK2 cDNA exhibits 93% and 90% sequence identity to human and mouse HIPK2, respectively.
- The deduced amino acid sequence shows high identity (98% with human, 96% with mouse) and conserved domain sites.
Conclusions:
- Canine HIPK2 is structurally highly similar to human and mouse HIPK2.
- These similarities suggest that canine HIPK2 likely possesses conserved biological functions.
- This finding supports the utility of canine models in studying HIPK2-related cancer mechanisms.
