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Extra Cellular Matrix-Based and Extra Cellular Matrix-Free Generation of Murine Testicular Organoids
Published on: October 7, 2020
[Culture and identification of human adult Leydig cells]
Xiang-Zhou Sun1, Chun-Hua Deng, Hai-Bin Guo
1Department of Urology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510080, China.
Objective:
To obtain a simple and effective method to isolate and purify adult Leydig cells.
Methods:
The testes of human adults were digested and then the density gradient centrifugation of the cells was performed with four different Percoll concentrations (60%, 34%, 26%, 21%) to isolate Leydig cells, whose characteristics were identified by cytological observation staining, 3beta-HSD staining and detection of hCG and testosterone secretion.
Results:
High-concentration (> 90%) purified Leydig cells were acquired, and many identification experiments demonstrated the adequate testosterone secretory function of the isolated and purified Leydig cells.
Conclusion:
This method is easy and efficient for the isolation and purification of adult Leydig cells.
