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Updated: Aug 11, 2026
Germ Cell Transplantation and Testis Tissue Xenografting in Mice
Published on: February 6, 2012
[Modified technique of testis autotransplantation in dogs]
Wei Zhang1, Ling-Long Wang, Jie Zhang
1Department of Urology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, China.
Objective:
To establish a dog model of testis autotransplantation with a modified technique.
Methods:
Testis autotransplantations were performed in 30 dogs. After detachment of the spermatic artery with a cuff of the abdominal aorta and the spermatic vein with a cuff of inferior vena cava, the testis was perfused and kept at ice temperature. An end-to-side anastomosis of the spermatic vessels to the external iliac vessels was conducted.
Results:
The success rate of the testis autotransplantations was 90% (27/30) and the time for heat ischemia, cold ischemia, anastomosis of spermatic vessels and the whole operation were (4.5 +/- 0.9) minutes, (50.0 +/- 5.0) minutes, (35.5 +/- 5.5) minutes and (3.5 +/- 0.5) hours respectively.
Conclusion:
A stable and feasible model of testis autotransplantation was established, which provides a reliable experimental base for testis autotransplantation.
