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Updated: Aug 17, 2026

Characterization of Calcification Events Using Live Optical and Electron Microscopy Techniques in a Marine Tubeworm
Published on: February 28, 2017
Microstructural characacterization of shell components in the mollusc Physa sp
Silvia M de Paula1, Marina Silveira
1Labroratory of Electron Microscopy, Institute of Physics, University of São Paulo, São Paulo, Brazil. sipaula@if.usp.br
Abstract:
Shells of the freshwater, pulmonate snail Physa (Mollusca, Gasteropoda), ranging from 0.5 to 10 mm in length, were studied using scanning microscopy, x-ray analysis, and infrared spectroscopy. Results obtained suggest that the shell is composed of aragonite, which occurs in several distinct crystalline forms. A selective distribution of crystalline forms (hexagonal plates, prisms, rhombohedra, and spherulites) occurred along specific sites of the shell. A variable distribution of the forms was also detected in adult shells and in protoconchs of developing embryos. Qualitative elemental analysis, using an energy-dispersive spectrometer, corroborates the presence of calcium, phosphorus and sulphur ions.

