Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Identification of plasmids by PCR-based replicon typing.

Alessandra Carattoli1, Alessia Bertini, Laura Villa

  • 1Department of Infectious, Parasitic and Immune-mediated Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy. alecara@iss.it

Journal of Microbiological Methods
|June 7, 2005
PubMed
Summary

A new PCR method identifies drug resistance plasmids in Enterobacteriaceae by targeting their replicons. This tool efficiently detects common plasmids in Salmonella, aiding in tracking multi-drug resistance spread.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evolutionary dynamics of the multidrug-resistant <i>Salmonella</i> Infantis harbouring the pESI megaplasmid across Europe.

Microbial genomics·2026
Same author

Response to "Skally et al. Surveillance of carbapenemase-producing organisms - considering the practical and local implications of current surveillance processes" [J Hosp Infect (2026) doi: 10.1016/j.jhin.2026.04.001].

The Journal of hospital infection·2026
Same author

Standardized numbering and alignment of the KPC family of β-lactamases.

Antimicrobial agents and chemotherapy·2026
Same author

The role of whole genome sequencing in antimicrobial susceptibility prediction of bacteria: 2025 update from the European Committee on Antimicrobial Susceptibility Testing Subcommittee.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2026
Same author

Genomic and molecular characterisation of a KPC-producing Klebsiella pneumoniae clinical isolate resistant to meropenem-vaborbactam, imipenem-relebactam, and ceftazidime-avibactam.

BMC genomic data·2026
Same author

SHARA-WoZ: A multistakeholder framework to evaluate socially assistive robots thought Wizard of the Oz methods.

Digital health·2026

Area of Science:

  • Microbiology
  • Molecular Biology
  • Epidemiology

Background:

  • Tracing plasmids that confer antibiotic resistance is crucial for understanding and controlling infectious diseases.
  • Enterobacteriaceae are a significant group of bacteria, often carrying resistance plasmids.
  • Existing methods for plasmid identification can be limited in scope or efficiency.

Purpose of the Study:

  • To develop and validate a Polymerase Chain Reaction (PCR) method for identifying major plasmid replicons within Enterobacteriaceae.
  • To assess the method's specificity and sensitivity using reference plasmids and clinical isolates.
  • To investigate the prevalence and distribution of specific plasmids in Salmonella enterica.

Main Methods:

  • Design of 18 primer pairs for multiplex and simplex PCR assays targeting 19 different plasmid incompatibility (inc) groups.

Related Experiment Videos

  • Testing the inc/rep PCR method on 61 reference plasmids to determine specificity.
  • Application of the method to 20 Salmonella enterica strains isolated in Italy to assess its performance on clinical samples.

    • Main Results:

    • The inc/rep PCR method demonstrated high specificity and sensitivity in detecting plasmid replicons.
    • The study identified recurrent and common plasmids among epidemiologically unrelated Salmonella isolates.
    • The method successfully characterized plasmids in diverse Salmonella serotypes.

    Conclusions:

    • The developed inc/rep PCR method is a valuable tool for identifying plasmid replicons in Enterobacteriaceae.
    • This method can effectively trace the dissemination of multi-drug resistance plasmids in various environments.
    • The findings highlight the utility of inc/rep PCR for epidemiological surveillance of antibiotic resistance.