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Updated: Aug 17, 2026

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
Published on: July 17, 2021
STR data for 15 loci in a population sample from the central region of Mexico
S Hernández-Gutiérrez1, P Hernández-Franco, S Martínez-Tripp
1Laboratorio de Biología Molecular, Universidad Panamericana, México DF, Mexico; Departamento de Medicina Genómica y Toxicología Ambiental (IIB-UNAM)(2), México DF, Mexico.
Abstract:
A Mexican population sample was obtained from the central region of the country, including five states. Two hundred and eleven individuals were PCR-typed for 15 STR loci with the AmpFiota STR Identifiler PCR amplification kit (Applied Biosystems). The following autosomal markers were analyzed: D8S1179, D21S11, D7S820, CSF1PO, D19S433, vWA, TPOX, D18S51, D3S1358, TH01, D13S317, D16S539, D2S1338, D5S818, FGA and Amelogenin. Allele frequencies for each STR were estimated and compared to previous reports. Genotype distribution by locus and by two loci combination was in agreement with Hardy-Weinberg expectations for all 15 STRs. This STR system in Mexican-mestizos presented a combined probability of exclusion (PE) and discrimination (PD) longer than 99.999%.
