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Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
A study of interlaboratory influence on column evaluation.
Cinzia Stella1, Sabine Heinisch, Xiangli Liu
1Laboratory of Pharmaceutical Analytical Chemistry, School of Pharmaceutical Sciences, EPGL, University of Geneva, Switzerland.
Journal of Pharmaceutical and Biomedical Analysis
|June 7, 2005
Summary
This study validated a liquid chromatography method for characterizing base deactivated columns across multiple labs. The findings confirm that consistent protocols allow for reliable comparison of chromatographic supports like Xterra RP 18 and Symmetry Shield.
Area of Science:
- Analytical Chemistry
- Chromatography Science
Background:
- Base deactivated columns are crucial in liquid chromatography.
- Standardized methods are needed for reliable column characterization.
- Inter-laboratory variability can affect chromatographic results.
Purpose of the Study:
- To investigate and validate a liquid chromatography (LC) method for characterizing base deactivated columns.
- To assess the comparability of chromatographic supports across different laboratories.
- To establish a robust protocol for inter-laboratory studies.
Main Methods:
- Collaborative study involving six laboratories.
- Utilized two chromatographic supports: Xterra RP 18 and Symmetry Shield.
- Investigated effects of cooling systems (water bath, air oven) and mobile phase preparation (by weight).
- Evaluated extra-column effects using neutral compounds.
Main Results:
- Column thermoregulation (water bath vs. air oven) did not significantly impact chromatographic data.
- Preparing mobile phase by weight improved control over composition.
- Extra-column effects were successfully evaluated in each laboratory.
- Chromatographic supports could be effectively compared when using the same test protocol across laboratories.
Conclusions:
- The developed liquid chromatography method is suitable for characterizing base deactivated columns.
- A standardized test protocol enables reliable inter-laboratory comparison of chromatographic supports.
- The study demonstrates the feasibility of harmonized analytical procedures in chromatography.
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