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Updated: Aug 17, 2026

Determination of the Gas-phase Acidities of Oligopeptides
Published on: June 24, 2013
Determination of sinomenine in human plasma by HPLC/ESI/ion trap mass spectrum
Yong-Mei Yao1, Zhi-Rong Tan, Zhe-Yi Hu
1Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Xiang-Ya School of Medicine, Central South University, Changsha, Hunan 410078, P.R. China.
Background:
Sinomenine is a pure alkaloid with a variety of pharmacological actions including anti-inflammation, immuno-suppression, analgesia, antihypertension, and antiarrhythmia. Methods have been developed to measure sinomenine in rats and rabbits through high-performance liquid chromatography (HPLC). But only one report described a method for determination of sinomenine in humans.
Method:
We developed a method to quantitate sinomenine in human plasma based on liquid chromatography-ion trap mass spectrometry coupled with electrospray ionization (HPLC/ESI/ion trap mass spectrum).
Results:
The calibration curve (r2=0.9993) of sinomenine was established with standard solutions. The mean inter-day and intra-day CVs<15%. The limit of detection for sinomenine was 0.5 ng/ml. The relative recovery of high, middle and low concentration was 99%, 103%, and 118%, respectively.
Conclusion:
The method is simple, rapid, sensitive, specific, and accurate enough for determination of sinomenine in human plasma and may be used to investigate the metabolism and pharmacokinetics of sinomenine.
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