Related Experiment Video
Updated: Aug 17, 2026

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
Published on: August 21, 2014
Global analysis of positive and negative pre-mRNA splicing regulators in Drosophila
Marco Blanchette1, Richard E Green, Steven E Brenner
1Department of Molecular and Cell Biology, Center for Integrative Genomics, Plant and Microbial Biology, University of California, Berkeley, 94720-3204 USA.
Abstract:
To gain insight into splicing regulation, we developed a microarray to assay all annotated alternative splicing events in Drosophila melanogaster and identified the alternative splice events controlled by four splicing regulators: dASF/SF2, B52/SRp55, hrp48, and PSI. The number of events controlled by each of these factors was found to be highly variable: dASF/SF2 strongly affects >300 splicing events, whereas PSI strongly affects only 43 events. Pairwise analysis also revealed many instances of splice site usage affected by multiple factors and provides the framework to understand the network controlling the alternatively spliced mRNA isoforms that compose the Drosophila transcriptome.
Related Concept Videos
Alternative RNA Splicing
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Chromatin Structure Regulates pre-mRNA Processing
The chromatin structure, especially...
Regulation of Expression at Multiple Steps
What is Gene Expression?
Pre-mRNA Processing: RNA Splicing
RNA Splicing

